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1x alphalisa buffer  (Revvity)


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    Revvity 1x alphalisa buffer
    Smaller antibody formats more effectively penetrate the human corneal epithelium and remain functional following penetration. ( A ) Schematic depiction of the 3D human epithelial corneal tissue model grown in cell culture inserts. IF8-Fc, db V2D7, and scFv V2D7 were applied topically on the corneal tissue layer at a concentration of 500 nM and left to penetrate for 6 h. ( B ) H&E-stained cross-section of 3D human corneal epithelial tissue model (upper image) and human corneal tissue (bottom image). AL: apical layer; WC: wing cells; BL: basal layer; M: microporous membrane; S: stroma ( C ) Percentage (%) of the antibody compared to the initial input or ( D ) antibody concentration (nM) that crossed the corneal epithelial layer after 6 h determined with an <t>AlphaLISA</t> assay. Representative plots of one out of two experiments performed. Mean of n = 3–8 replicates. ( E ) Human corneal epithelial cells express ALCAM, as assessed by flow cytometry (n = 1). ( F ) Transepithelial electrical resistance (TEER) of the 3D corneal tissue model measured after the penetration assay. Representative plot of one out of two experiments performed. Mean of n = 4 replicates. ( G ) Schematic depiction of the dendritic cell (DC) transmigration assay performed in transwells containing a lymphatic endothelial cell (LEC) monolayer. The medium from the penetration assay containing mAbs that crossed the corneal epithelial layer was applied to the top of the transwell to assess its ability to block DC transmigration towards the chemokine CCL21. ( H ) DC transmigration performed in the presence of the medium collected from the corneal tissue penetration assays (see A – D ) with scFv, db and IF8-Fc (one technical replicate out of two shown) or negative control PBS (n = four transwells per condition). IF8-Fc at 1 nM was used as a positive control, and PBS was used as the negative control (control). Representative plot of one out of two experiments. Statistics: One-way ANOVA, Holm–Sidak multiple comparison test ( C , D ) or Dunnett’s multiple comparison test ( H ).
    1x Alphalisa Buffer, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1x+alphalisa+buffer/Immunoassay+buffer%2C+10X/pmc10385607-181-11-15
    Average 91 stars, based on 10 article reviews
    1x alphalisa buffer - by Bioz Stars, 2026-10
    91/100 stars

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    1) Product Images from "Optimization and Characterization of Novel ALCAM-Targeting Antibody Fragments for Transepithelial Delivery"

    Article Title: Optimization and Characterization of Novel ALCAM-Targeting Antibody Fragments for Transepithelial Delivery

    Journal: Pharmaceutics

    doi: 10.3390/pharmaceutics15071841

    Smaller antibody formats more effectively penetrate the human corneal epithelium and remain functional following penetration. ( A ) Schematic depiction of the 3D human epithelial corneal tissue model grown in cell culture inserts. IF8-Fc, db V2D7, and scFv V2D7 were applied topically on the corneal tissue layer at a concentration of 500 nM and left to penetrate for 6 h. ( B ) H&E-stained cross-section of 3D human corneal epithelial tissue model (upper image) and human corneal tissue (bottom image). AL: apical layer; WC: wing cells; BL: basal layer; M: microporous membrane; S: stroma ( C ) Percentage (%) of the antibody compared to the initial input or ( D ) antibody concentration (nM) that crossed the corneal epithelial layer after 6 h determined with an AlphaLISA assay. Representative plots of one out of two experiments performed. Mean of n = 3–8 replicates. ( E ) Human corneal epithelial cells express ALCAM, as assessed by flow cytometry (n = 1). ( F ) Transepithelial electrical resistance (TEER) of the 3D corneal tissue model measured after the penetration assay. Representative plot of one out of two experiments performed. Mean of n = 4 replicates. ( G ) Schematic depiction of the dendritic cell (DC) transmigration assay performed in transwells containing a lymphatic endothelial cell (LEC) monolayer. The medium from the penetration assay containing mAbs that crossed the corneal epithelial layer was applied to the top of the transwell to assess its ability to block DC transmigration towards the chemokine CCL21. ( H ) DC transmigration performed in the presence of the medium collected from the corneal tissue penetration assays (see A – D ) with scFv, db and IF8-Fc (one technical replicate out of two shown) or negative control PBS (n = four transwells per condition). IF8-Fc at 1 nM was used as a positive control, and PBS was used as the negative control (control). Representative plot of one out of two experiments. Statistics: One-way ANOVA, Holm–Sidak multiple comparison test ( C , D ) or Dunnett’s multiple comparison test ( H ).
    Figure Legend Snippet: Smaller antibody formats more effectively penetrate the human corneal epithelium and remain functional following penetration. ( A ) Schematic depiction of the 3D human epithelial corneal tissue model grown in cell culture inserts. IF8-Fc, db V2D7, and scFv V2D7 were applied topically on the corneal tissue layer at a concentration of 500 nM and left to penetrate for 6 h. ( B ) H&E-stained cross-section of 3D human corneal epithelial tissue model (upper image) and human corneal tissue (bottom image). AL: apical layer; WC: wing cells; BL: basal layer; M: microporous membrane; S: stroma ( C ) Percentage (%) of the antibody compared to the initial input or ( D ) antibody concentration (nM) that crossed the corneal epithelial layer after 6 h determined with an AlphaLISA assay. Representative plots of one out of two experiments performed. Mean of n = 3–8 replicates. ( E ) Human corneal epithelial cells express ALCAM, as assessed by flow cytometry (n = 1). ( F ) Transepithelial electrical resistance (TEER) of the 3D corneal tissue model measured after the penetration assay. Representative plot of one out of two experiments performed. Mean of n = 4 replicates. ( G ) Schematic depiction of the dendritic cell (DC) transmigration assay performed in transwells containing a lymphatic endothelial cell (LEC) monolayer. The medium from the penetration assay containing mAbs that crossed the corneal epithelial layer was applied to the top of the transwell to assess its ability to block DC transmigration towards the chemokine CCL21. ( H ) DC transmigration performed in the presence of the medium collected from the corneal tissue penetration assays (see A – D ) with scFv, db and IF8-Fc (one technical replicate out of two shown) or negative control PBS (n = four transwells per condition). IF8-Fc at 1 nM was used as a positive control, and PBS was used as the negative control (control). Representative plot of one out of two experiments. Statistics: One-way ANOVA, Holm–Sidak multiple comparison test ( C , D ) or Dunnett’s multiple comparison test ( H ).

    Techniques Used: Functional Assay, Cell Culture, Concentration Assay, Staining, Membrane, Flow Cytometry, Transmigration Assay, Blocking Assay, Negative Control, Positive Control, Control, Comparison

    Related Articles

    Functional Assay:

    Article Title: Optimization and Characterization of Novel ALCAM-Targeting Antibody Fragments for Transepithelial Delivery
    Article Snippet: AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).. In brief, 10 µL of 10 nM hALCAM V1 diluted in 1X AlphaLISA buffer (AL000C, PerkinElmer) was added to wells of a 96-well plate (6002350, PerkinElmer).. For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).

    Cell Culture:

    Article Title: Optimization and Characterization of Novel ALCAM-Targeting Antibody Fragments for Transepithelial Delivery
    Article Snippet: AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).. In brief, 10 µL of 10 nM hALCAM V1 diluted in 1X AlphaLISA buffer (AL000C, PerkinElmer) was added to wells of a 96-well plate (6002350, PerkinElmer).. For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).

    Concentration Assay:

    Article Title: Optimization and Characterization of Novel ALCAM-Targeting Antibody Fragments for Transepithelial Delivery
    Article Snippet: AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).. In brief, 10 µL of 10 nM hALCAM V1 diluted in 1X AlphaLISA buffer (AL000C, PerkinElmer) was added to wells of a 96-well plate (6002350, PerkinElmer).. For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).

    Staining:

    Article Title: Optimization and Characterization of Novel ALCAM-Targeting Antibody Fragments for Transepithelial Delivery
    Article Snippet: AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).. In brief, 10 µL of 10 nM hALCAM V1 diluted in 1X AlphaLISA buffer (AL000C, PerkinElmer) was added to wells of a 96-well plate (6002350, PerkinElmer).. For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).

    Membrane:

    Article Title: Optimization and Characterization of Novel ALCAM-Targeting Antibody Fragments for Transepithelial Delivery
    Article Snippet: AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).. In brief, 10 µL of 10 nM hALCAM V1 diluted in 1X AlphaLISA buffer (AL000C, PerkinElmer) was added to wells of a 96-well plate (6002350, PerkinElmer).. For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).

    Flow Cytometry:

    Article Title: Optimization and Characterization of Novel ALCAM-Targeting Antibody Fragments for Transepithelial Delivery
    Article Snippet: AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).. In brief, 10 µL of 10 nM hALCAM V1 diluted in 1X AlphaLISA buffer (AL000C, PerkinElmer) was added to wells of a 96-well plate (6002350, PerkinElmer).. For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).

    Transmigration Assay:

    Article Title: Optimization and Characterization of Novel ALCAM-Targeting Antibody Fragments for Transepithelial Delivery
    Article Snippet: AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).. In brief, 10 µL of 10 nM hALCAM V1 diluted in 1X AlphaLISA buffer (AL000C, PerkinElmer) was added to wells of a 96-well plate (6002350, PerkinElmer).. For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).

    Blocking Assay:

    Article Title: Optimization and Characterization of Novel ALCAM-Targeting Antibody Fragments for Transepithelial Delivery
    Article Snippet: AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).. In brief, 10 µL of 10 nM hALCAM V1 diluted in 1X AlphaLISA buffer (AL000C, PerkinElmer) was added to wells of a 96-well plate (6002350, PerkinElmer).. For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).

    Negative Control:

    Article Title: Optimization and Characterization of Novel ALCAM-Targeting Antibody Fragments for Transepithelial Delivery
    Article Snippet: AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).. In brief, 10 µL of 10 nM hALCAM V1 diluted in 1X AlphaLISA buffer (AL000C, PerkinElmer) was added to wells of a 96-well plate (6002350, PerkinElmer).. For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).

    Positive Control:

    Article Title: Optimization and Characterization of Novel ALCAM-Targeting Antibody Fragments for Transepithelial Delivery
    Article Snippet: AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).. In brief, 10 µL of 10 nM hALCAM V1 diluted in 1X AlphaLISA buffer (AL000C, PerkinElmer) was added to wells of a 96-well plate (6002350, PerkinElmer).. For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).

    Control:

    Article Title: Optimization and Characterization of Novel ALCAM-Targeting Antibody Fragments for Transepithelial Delivery
    Article Snippet: AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).. In brief, 10 µL of 10 nM hALCAM V1 diluted in 1X AlphaLISA buffer (AL000C, PerkinElmer) was added to wells of a 96-well plate (6002350, PerkinElmer).. For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).

    Comparison:

    Article Title: Optimization and Characterization of Novel ALCAM-Targeting Antibody Fragments for Transepithelial Delivery
    Article Snippet: AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).AlphaLISA was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA).. In brief, 10 µL of 10 nM hALCAM V1 diluted in 1X AlphaLISA buffer (AL000C, PerkinElmer) was added to wells of a 96-well plate (6002350, PerkinElmer).. For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).For the standard curves, dilutions of antibodies (IF8-Fc, diabody V2D7, scFv V2D7) were prepared in COR-100 media (final concentrations 15 nM to 0.23 nM, 1:2 dilutions).



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    1x alphalisa buffer  (Revvity)
    91
    Revvity 1x alphalisa buffer
    Smaller antibody formats more effectively penetrate the human corneal epithelium and remain functional following penetration. ( A ) Schematic depiction of the 3D human epithelial corneal tissue model grown in cell culture inserts. IF8-Fc, db V2D7, and scFv V2D7 were applied topically on the corneal tissue layer at a concentration of 500 nM and left to penetrate for 6 h. ( B ) H&E-stained cross-section of 3D human corneal epithelial tissue model (upper image) and human corneal tissue (bottom image). AL: apical layer; WC: wing cells; BL: basal layer; M: microporous membrane; S: stroma ( C ) Percentage (%) of the antibody compared to the initial input or ( D ) antibody concentration (nM) that crossed the corneal epithelial layer after 6 h determined with an <t>AlphaLISA</t> assay. Representative plots of one out of two experiments performed. Mean of n = 3–8 replicates. ( E ) Human corneal epithelial cells express ALCAM, as assessed by flow cytometry (n = 1). ( F ) Transepithelial electrical resistance (TEER) of the 3D corneal tissue model measured after the penetration assay. Representative plot of one out of two experiments performed. Mean of n = 4 replicates. ( G ) Schematic depiction of the dendritic cell (DC) transmigration assay performed in transwells containing a lymphatic endothelial cell (LEC) monolayer. The medium from the penetration assay containing mAbs that crossed the corneal epithelial layer was applied to the top of the transwell to assess its ability to block DC transmigration towards the chemokine CCL21. ( H ) DC transmigration performed in the presence of the medium collected from the corneal tissue penetration assays (see A – D ) with scFv, db and IF8-Fc (one technical replicate out of two shown) or negative control PBS (n = four transwells per condition). IF8-Fc at 1 nM was used as a positive control, and PBS was used as the negative control (control). Representative plot of one out of two experiments. Statistics: One-way ANOVA, Holm–Sidak multiple comparison test ( C , D ) or Dunnett’s multiple comparison test ( H ).
    1x Alphalisa Buffer, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1x+alphalisa+buffer/Immunoassay+buffer%2C+10X/pmc10385607-181-11-15
    Average 91 stars, based on 1 article reviews
    1x alphalisa buffer - by Bioz Stars, 2026-10
    91/100 stars
    		  Buy from Supplier

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    Smaller antibody formats more effectively penetrate the human corneal epithelium and remain functional following penetration. ( A ) Schematic depiction of the 3D human epithelial corneal tissue model grown in cell culture inserts. IF8-Fc, db V2D7, and scFv V2D7 were applied topically on the corneal tissue layer at a concentration of 500 nM and left to penetrate for 6 h. ( B ) H&E-stained cross-section of 3D human corneal epithelial tissue model (upper image) and human corneal tissue (bottom image). AL: apical layer; WC: wing cells; BL: basal layer; M: microporous membrane; S: stroma ( C ) Percentage (%) of the antibody compared to the initial input or ( D ) antibody concentration (nM) that crossed the corneal epithelial layer after 6 h determined with an AlphaLISA assay. Representative plots of one out of two experiments performed. Mean of n = 3–8 replicates. ( E ) Human corneal epithelial cells express ALCAM, as assessed by flow cytometry (n = 1). ( F ) Transepithelial electrical resistance (TEER) of the 3D corneal tissue model measured after the penetration assay. Representative plot of one out of two experiments performed. Mean of n = 4 replicates. ( G ) Schematic depiction of the dendritic cell (DC) transmigration assay performed in transwells containing a lymphatic endothelial cell (LEC) monolayer. The medium from the penetration assay containing mAbs that crossed the corneal epithelial layer was applied to the top of the transwell to assess its ability to block DC transmigration towards the chemokine CCL21. ( H ) DC transmigration performed in the presence of the medium collected from the corneal tissue penetration assays (see A – D ) with scFv, db and IF8-Fc (one technical replicate out of two shown) or negative control PBS (n = four transwells per condition). IF8-Fc at 1 nM was used as a positive control, and PBS was used as the negative control (control). Representative plot of one out of two experiments. Statistics: One-way ANOVA, Holm–Sidak multiple comparison test ( C , D ) or Dunnett’s multiple comparison test ( H ).

    Journal: Pharmaceutics

    Article Title: Optimization and Characterization of Novel ALCAM-Targeting Antibody Fragments for Transepithelial Delivery

    doi: 10.3390/pharmaceutics15071841

    Figure Lengend Snippet: Smaller antibody formats more effectively penetrate the human corneal epithelium and remain functional following penetration. ( A ) Schematic depiction of the 3D human epithelial corneal tissue model grown in cell culture inserts. IF8-Fc, db V2D7, and scFv V2D7 were applied topically on the corneal tissue layer at a concentration of 500 nM and left to penetrate for 6 h. ( B ) H&E-stained cross-section of 3D human corneal epithelial tissue model (upper image) and human corneal tissue (bottom image). AL: apical layer; WC: wing cells; BL: basal layer; M: microporous membrane; S: stroma ( C ) Percentage (%) of the antibody compared to the initial input or ( D ) antibody concentration (nM) that crossed the corneal epithelial layer after 6 h determined with an AlphaLISA assay. Representative plots of one out of two experiments performed. Mean of n = 3–8 replicates. ( E ) Human corneal epithelial cells express ALCAM, as assessed by flow cytometry (n = 1). ( F ) Transepithelial electrical resistance (TEER) of the 3D corneal tissue model measured after the penetration assay. Representative plot of one out of two experiments performed. Mean of n = 4 replicates. ( G ) Schematic depiction of the dendritic cell (DC) transmigration assay performed in transwells containing a lymphatic endothelial cell (LEC) monolayer. The medium from the penetration assay containing mAbs that crossed the corneal epithelial layer was applied to the top of the transwell to assess its ability to block DC transmigration towards the chemokine CCL21. ( H ) DC transmigration performed in the presence of the medium collected from the corneal tissue penetration assays (see A – D ) with scFv, db and IF8-Fc (one technical replicate out of two shown) or negative control PBS (n = four transwells per condition). IF8-Fc at 1 nM was used as a positive control, and PBS was used as the negative control (control). Representative plot of one out of two experiments. Statistics: One-way ANOVA, Holm–Sidak multiple comparison test ( C , D ) or Dunnett’s multiple comparison test ( H ).

    Article Snippet: In brief, 10 μL of 10 nM hALCAM V1 diluted in 1X AlphaLISA buffer (AL000C, PerkinElmer) was added to wells of a 96-well plate (6002350, PerkinElmer).

    Techniques: Functional Assay, Cell Culture, Concentration Assay, Staining, Membrane, Flow Cytometry, Transmigration Assay, Blocking Assay, Negative Control, Positive Control, Control, Comparison